Pack, Mandy and Götz, Claudia and Wrublewsky, Selina and Montenarh, Mathias (2021) SGC-CK2-1 Is an Efficient Inducer of Insulin Production and Secretion in Pancreatic β-Cells. Pharmaceutics, 14 (1). p. 19. ISSN 1999-4923
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Abstract
The pyrazolopyrimidine based compound SGC-CK2-1 is a potent and highly specific CK2 inhibitor and a new tool to study the biological functions of protein kinase CK2 irrespective from off-target effects. We used this compound in comparison with the well-established CK2 inhibitor CX-4945 to analyze the importance of CK2 for insulin production and secretion from pancreatic β-cells. Both inhibitors affected the proliferation and viability of MIN6 cells only marginally and downregulated the endogenous CK2 activity to a similar level. Furthermore, both inhibitors increased the message for insulin and boosted the secretion of insulin from storage vesicles. Thus, regarding the high specificity of SGC-CK2-1, we can clearly attribute the observed effects to biological functions of protein kinase CK2.
Item Type: | Article |
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Uncontrolled Keywords: | protein kinase CK2; SGC-CK2-1; CX-4945; pancreatic β-cells; insulin |
Subjects: | Institute Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 11 Nov 2022 04:38 |
Last Modified: | 30 Dec 2023 13:08 |
URI: | http://eprint.subtopublish.com/id/eprint/95 |