Kim, Jiyoon and Cho, Siwoo and Park, Yonghyun and Lee, Jiyoul and Park, Jaesung and Fabbri, Muller (2021) Evaluation of micro-RNA in extracellular vesicles from blood of patients with prostate cancer. PLOS ONE, 16 (12). e0262017. ISSN 1932-6203
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Abstract
Extracellular vesicles (EVs) contain various types of molecules including micro-RNAs, so isolating EVs can be an effective way to analyze and diagnose diseases. A lot of micro-RNAs have been known in relation to prostate cancer (PCa), and we evaluate miR-21, miR-141, and miR-221 in EVs and compare them with prostate-specific antigen (PSA). EVs were isolated from plasma of 38 patients with prostate cancer and 8 patients with benign prostatic hyperplasia (BPH), using a method that showed the highest recovery of RNA. Isolation of EVs concentrated micro-RNAs, reducing the cycle threshold (Ct) value of RT-qPCR amplification of micro-RNA such as miR-16 by 5.12 and miR-191 by 4.65, compared to the values before EV isolation. Normalization of target micro-RNAs was done using miR-191. For miR-221, the mean expression level of patients with localized PCa was significantly higher than that of the control group, having 33.45 times higher expression than the control group (p < 0.01). Area under curve (AUC) between BPH and PCa for miR-221 was 0.98 (p < 0.0001), which was better than AUC for prostate-specific antigen (PSA) level in serum for the same patients. The levels of miR-21 and miR-141 in EVs did not show significant changes in patients with PCa compared to the control group in this study. This study suggests isolating EVs can be a helpful approach in analyzing micro-RNAs with regard to disease.
Item Type: | Article |
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Subjects: | Institute Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 28 Feb 2023 08:46 |
Last Modified: | 17 Jul 2024 07:17 |
URI: | http://eprint.subtopublish.com/id/eprint/710 |