IDENTIFICATION OF PHENOLIC COMPOUNDS FROM THE LEAVES EXTRACT OF Clinacanthus nutans LINDAU

Clinacanthus nutans (C. nutans) lindau isone of the significant members of the Acanthaceaefamily. C. nutanshasattracted more attention from various researchers because of its medicinal values. This plant has a wide range of bioactive compounds.The HPLC technique was used for the immediate determination of flavonoids (rutin, gallic acid, quercetin, Kaemferol and ascorbic acid) in the leafextract of C.nutans. Anultrasound-assisted extraction was used for the pretreatment processof a sample with methanol by using a temperature of 50◦C for 40 min at 35 kHz, followed by separation on a C18 column and usedwater: methanol: acetonitrile (45:40:15 v/v/v) containing acetic acid 1.0% as a mobile phase. A 254nm wavelength was used to detect the extract.The standard retention timesof the gallic acid, rutin, ascorbic acid, quercetin and kaempferol were found to be at 2.610, 2.875, 3.150, 5.789 and,8.983 min, respectively. The presence of gallic acid, rutin, ascorbic acid, kaempferol and quercetin in the C. nutans leaf extract was found to match according to the standard retention time. In the C. nutans leaves,the gallic acid was found to have a retention time of 2.550 min, rutin of 2.865 min, quercetin of5.798 min and kaempferol was found to have a retention time of 8.984 min. Whereas, ascorbic acid was not identified. The gallic acid and rutin peaks were a little bit merged; however,they showed different retention times.