COMPARATIVE OF PCR SUCCESS WITH CHLOROPLAST MARKERS FOR BARCODING IN SELECTED FORESTRY SPECIES

Chloroplast markers study is an approach applied for identifying molecular markers via DNA barcode. The chloroplast marker, rbcL, and matK are utilized in DNA barcoding and phylogenetic analysis. This study utilized chloroplast markers to identify some forestry species. The species evaluated in this study were Aleurites moluccana (Euphorbiaceae), Arenga pinnata (Arecaceae), Bambusa sp. (Poaceae), Neolamarckia macrophylla (Rubiaceae), and Vatica sp. (Dipterocarpaceae).

The amplification success rate of rbcL markers was higher than the matK marker. The chloroplast markers are matK (matK 4, matK 5) and rbcL (rbcL1, rbcL 2, rbcL 3, and rbcL 4), which have been successfully amplified in this study, can be recommended as chloroplast markers in the following studies and used to identify species. This study indicates that the chloroplast markers, as universal primers, identified the five evaluated plant families. Therefore, it is necessary to carry out sequencing analysis using other matK and rbcL primers, which have been already reported, can amplify other plant families for strengthening and supporting the genetic information and species morphological identification.