Sampling of Cocoa Beans and Quantification of Ochratoxin A: Validation of the Methods

The objectives of this study were compare an alternative method for cocoa beans sampling with the
standard method proposed by the European Union (EC 401/2006) and validate a method of
Ochratoxin A determination. The alternative method applies to samples of 5 kg of cocoa beans while
the standard method applies to samples of 10 kg. quality characteristics and validation parameters
were determined according to Ivorian Coffee and Cocoa stock exchange and French (NFV03-110-
1998) standards. Concerning quality characteristics, no significant difference at 5% risk was revealed
in the values of the three parameters considered when assessing marketability quality requirements
(moisture, graining and grades). As regards the validation of OTA determination method, the limits of
detections and quantifications were 0.05 μg/kg and 0.20 μg/kg. The coefficients of variation for the
tests of repeatability and reproducibility were respectively 0.26% and 5.67%. As for the extraction
yield, it was equal to 86%. Furthermore, no significant difference (5% risk) was observed between the
concentrations of OTA measured by the standard and alternative methods. Hence, although the
alternative method goes with a mass reduction of samples analyzed, it did not alter significantly the
results of the marketability as well as the concentrations of OTA