Li, Ling and Huang, Jungang and Zhao, Zongkai and Wen, Zhuzhi and Li, Kang and Ma, Tianjiao and Zhang, Lisui and Zheng, Junmeng and Liang, Shi and Zhou, Hao (2021) Decreased Spp1 Expression in Acute Myocardial Infarction after Ischemia and Reperfusion Injury. Cardiology Research and Practice, 2021. pp. 1-8. ISSN 2090-8016
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Abstract
Background. With the progress of shock therapy and the establishment and promotion of methods such as thrombolytic therapy and percutaneous coronary intervention (PCI), many tissues and organs have been reperfused after ischemia which may cause even worse disorder called ischemia-reperfusion injury (IRI). mRNAs have been found to have significant impacts on ischemia-reperfusion through various mechanisms. In view of the accessibility of mRNAs from blood, we aimed to find the association between mRNA and ischemia-reperfusion. Methods. We used the GSE83472 dataset from the Gene Expression Omnibus (GEO) database to find differential RNA expression between ischemia-reperfusion tissue and control samples. In addition, Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed to find the biological property of 449 RNAs from GSE83472 via the Database for Annotation, Visualization, and Integrated Discovery (DAVID). Besides, Gene Set Enrichment Analysis (GSEA), a tool to find the pathway orientation of a gene set, was used for further study in the four most significant KEGG pathways. Furthermore, we constructed a protein-protein interaction (PPI) network. In the end, we used quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and western blotting to measure and compare the expression of Spp1 in patients who accepted percutaneous coronary intervention. Results. The bioinformatics analyses suggested that Spp1 was a hub gene in reperfusion after ischemia. The qRT-PCR result showed that the Spp1 expression was significantly downregulated in ischemia-reperfusion cells after PCI compared with normal samples and so as the western blotting. Conclusion. Spp1 might play an essential role in acute myocardial infarction after ischemia and reperfusion injury.
Item Type: | Article |
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Subjects: | Institute Archives > Medical Science |
Depositing User: | Managing Editor |
Date Deposited: | 19 Nov 2022 03:44 |
Last Modified: | 22 Feb 2024 03:50 |
URI: | http://eprint.subtopublish.com/id/eprint/341 |