Inhibitory Effects of Grape Seeds Powder, Extract and Oil on Gentamicin Induced Nephrotoxicity in Rats

This study was designed to determine the Inhibitory effect of grape seeds powder, extract and oil on gentamicin-induced nephrotoxicity in rats.The study was carried out on 30 adult albino male rats Sprague-Dawley strain were classified into two groups: The first group (n= 6) left as normal control group(-ve), fed on basal diet, the second group(n= 24) injected with gentamicin (100 mg/kg/day for 7 days i.p). These animals were divided into 4 groups 6 rats each of these , control (+ve) group and 3 groups were treated with grape seeds powder ( 10 % of diet ) , grape seeds extract (300mg/kg b.w.orally by stomach tube) and grape seeds oil (4 ml/kg b.w. orally by stomach tube) respectively. The treatment period was designed for eight weeks. The results revealed that, Gentamicin injection induced marked nephrotoxicity as evidenced by significant elevation in serum levels of creatinine, urea, uric acid, A/G ratio, MDA ,renin hormone, phosphorus and potassium, with significant reduction in serum levels of total protein, albumin, globulin, (SOD), (GPX), (GST) ,catalase antioxidant enzymes, erythropoietin hormone (EPO) , vitamin D and Ca . While the rat groups treated with grape seeds powder ,extract and oil showed significant improvement of the levels of serum total protein, albumin, globulin, kidney tissue superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione transferase (GST) , catalase antioxidant enzymes, erythropoietin hormone (EPO) and vitamin D, and significant decrease in serum creatinine, urea, uric acid, albumin/globulin ratio ,kidney tissue malondialdehyde (MDA), renin hormone, phosphorus and potassium compared with control(+ve) group. In conclusion, feeding rats with grape seeds powder ,extract and oil may significantly reduce nephrotoxicity, So this study recommend the use of grape seeds especially grape seeds oil or grape seeds extract for humans suffering nephrotoxicity, could be of value.