Development of Validated RP-HPLC Method for Simultanious Estimation of Cefadroxil and Ambroxol in Pure and Pharmaceutical Dosage Form

A precise, accurate, economical and simple HPLC (reverse phase) method was developed for the estimation of Cefadroxil and Ambroxol in bulk and pharmaceutical dosage form. Method was performed on a octadecyl silane column with dimensions 4.6 x 250 mm having particle size 5 micron. The mobile phase used in the method is methanol: phosphate buffer pH 3 (60:40v/v). The rate of flow was maintained at 1.5 ml/ min and effluent was monitored at 243 nm. Retention times were observed 2.3 min and 3.3 min for CFX and AXL respectively. The standard calibration curve was found linear over a range of 16–80 µg/ml and 2-10 µg/ml for CFX and AXL respectively. Similarly an average correlation coefficient was also obtained at 0.999 for CFX and AXL. LOQ limit was found at 2 µg/ml and absolute recovery was found 99.6% and 99.5% for CFX and AXL respectively. This method can be utilized for routine analysis of CFX and AXL in pure and dosage form.