Induction of Systemic Resistant Molecules in Phylloplane of Rice Plants against Magnaporthe oryzae by Pseudomonas fluorescens

Rice blast caused by ascomycetes fungus Magnaporthe oryzae, is mostly considered the utmost significant disease of rice worldwide since its widespread dissemination and destructiveness under conducive conditions. Experiments were conducted in phylloplane of rice plants to study the induction of various defense enzymes and accumulation of phenol by three biocontrol agents viz., P. fluorescens talc (Pf1), liquid formulation with TNAU - Pf1 and Biocure - B under pot culture. The application of bioformulatons of P. flourescens triggered the activity of three defense related enzymes viz., peroxidase (PO), polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) and SOD were induced and the accumulation of phenol was also noticed in phylloplane rice plant upon challenge inoculation with Magnaporthe oryzae the causal agent for leaf blast disease in rice. The activities of defense enzymes reached a peak at three days after inoculation (DAI) with the pathogen. Native PAGE analysis revealed the expression of an additional isoforms of PO, PPO, SOD and catalase were observed in biocontrol agents treated seedlings due to induced systemic resistance (ISR) induction.