Therapeutic Potential of Parkia biglobosa Seed against Potassium Bromate-induced Testicular Toxicity

Ezirim, E.O. and Uche, C.L. and Abali, I. O. and Iwuoha, C. E. and Chika-Igwenyi, N. M. and Onyeaghala, C. A. and Orji, S. F. and Ugwu, C. N. and Ugwu, N. I. and Igwenyi, C. and Airaodion, A.I. (2022) Therapeutic Potential of Parkia biglobosa Seed against Potassium Bromate-induced Testicular Toxicity. International Journal of Research and Reports in Gynaecology, 5 (3). pp. 78-89.

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Abstract

Aim: This study was aimed at assessing the therapeutic potential of Parkia biglobosa (P. biglobosa) seed against potassium bromate-induced testicular toxicity.

Methodology: P. biglobosa was extracted with soxhlet extractor with ethanol as the solvent. Twenty-four adult male Wistar rats were acclimatized under laboratory conditions and were randomly grouped into A, B, C and D. Group A was given distilled water orally. Animals in groups B, C and D were administered 100 mg/kg body weight of potassium bromate, but groups C and D were also treated with 100 and 200 mg/kg body weight of P. biglobosa respectively. Both potassium bromate and P. biglobosa were freshly prepared on daily basis and administered to rats by oral gavage. After 28 days of treatment, the animals were sacrificed under mild diethyl ether anaesthetization 24 hours after cessation of last treatment. The testes were removed homogenized in the ice cold 0.25 M sucrose solution. The homogenates were centrifuged at 5000 ×g for 10 minutes in a refrigerated centrifuge. The supernatant was collected and stored frozen for further analysis. The parameters were measured using standard methods.

Results: When compared to animals in the control group, animals intoxicated with KBrO3 had lower testicular concentrations of total cholesterol, total protein, glycogen, sialic acid, MDA, and GSH, as well as higher levels of ALP, SOD, and CAT activity. Additionally, it was shown that as compared to the animals in the control group, KBrO3 boosted the testicular ACP's activity. However, P. biglobosa treatment of intoxicated rats reduced these alterations in a dose-dependent manner.

Conclusion: The results of this investigation demonstrated that potassium bromate caused testicular toxicity, and that P. biglobosa treatment counteracted this effect. Thus, it is recommended that these results be investigated in clinical trials in human volunteers.

Item Type: Article
Subjects: Institute Archives > Medical Science
Depositing User: Managing Editor
Date Deposited: 08 Mar 2023 07:01
Last Modified: 08 Mar 2024 04:05
URI: http://eprint.subtopublish.com/id/eprint/1782

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